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Image Search Results
Journal: Frontiers in Immunology
Article Title: Single-cell sequencing decodes the secrets of the RAP phenomenon of corticotomy
doi: 10.3389/fimmu.2024.1397727
Figure Lengend Snippet: Pseudotime and SCENIC analyses revealed that Atf3 regulates a new direction for osteoclast differentiation (A) The monocytes_macrophages developmental trajectory was determined using Monocle. (B) A heat map of DEGs was made based on their simple kinetics through pseudotime using Dynverse and KEGG enrichment of each module. (C) The pseudotime expression trend diagram of osteoclast-related genes (Acp5, Ctsk, Mmp9, Dcstamp, Ocstamp, and Atp6v0d2) using pseudotime analysis; (D) A heat map of differentially expressed regulons in each monocyte_macrophage and the regulon specificity ranking diagram of each monocyte_macrophage. (E) ICC of CD68 and Atf3 and TRAP staining of osteoclasts in the control and corticotomy groups (white arrow indicates Atf3 and black arrow indicates the corresponding osteoclast).
Article Snippet: To perform TRAP staining, the samples were fixed with the
Techniques: Expressing, Staining, Control